Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins
Identifieur interne : 000645 ( Main/Exploration ); précédent : 000644; suivant : 000646Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins
Auteurs : Roy M. Pemberton [Royaume-Uni] ; Frankie J. Rawson [Royaume-Uni] ; JINSHENG XU [Royaume-Uni] ; Robin Pittson [Royaume-Uni] ; Guido A. Drago [Royaume-Uni] ; John Griffiths [Royaume-Uni] ; Simon K. Jackson [Royaume-Uni] ; John P. Hart [Royaume-Uni]Source :
- Mikrochimica acta : (1966. Print) [ 0026-3672 ] ; 2010.
Descripteurs français
- Pascal (Inist)
- Application, Biodétecteur, Environnement, Toxine, Carbone, Cobalt, Enzyme, Solution tampon, Voltammétrie cyclique, Densité élevée, Régime permanent, Ampérométrie, Diffusion, Microélectrode, Eau, Encre, Phtalocyanine, Glucose oxidase, Lactate, Hexacyanoferrate II, Phosphate, Utilisation, Platine, Milieu culture, Sensibilité, Dynamique, Temps, Libération, Benzène(1,3-dinitro), Réduction, Production, Culture cellulaire, Toxicité, Monitorage.
- Wicri :
English descriptors
- KwdEn :
- Amperometry, Application, Biosensor, Buffer solution, Carbon, Cell culture, Cobalt, Culture medium, Cyclic voltammetry, Diffusion, Dynamics, Environment, Enzyme, Glucose oxidase, Hexacyanoferrates II, High density, Ink, Lactates, Microelectrode, Monitoring, Phosphates, Phthalocyanine, Platinum, Production, Reduction, Release, Sensitivity, Steady state, Time, Toxicity, Toxin, Use, Water.
Abstract
Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferrocyanide and H2O2 in phosphate buffer solution: sigmoidal cyclic voltammograms, high current density values and steady-state amperometric responses confirmed the existence of radial-diffusion-limiting microelectrode behaviour. The lactate microband biosensors were then used, in conjunction with a screen-printed Ag/AgCl reference and platinum counter electrode, to monitor lactate levels in culture medium, with a linear range of 0.5-5 mM, sensitivity of 20 nA.mM-1, and dynamic range up to >9 mM. The lactate microband biosensors could operate continuously in culture medium over extended times (up to 24 h) at 37°C. These biosensors were then applied to detect changes in lactate release from cultured cells in response to toxic challenge: m-dinitrobenzene (500 μM) caused a reduction in lactate production by high-passage number HepG2 single cells; D-galactosamine (20 mM) induced release of lactate by HepG2 spheroid cultures. This novel use of microband biosensors in cell culture has the potential for further application in toxicity monitoring, in both environmental and pharmaceutical areas.
Affiliations:
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Drago</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Applied Enzyme Technology Ltd., Monmouth House, Mamhilad Business Park</s1><s2>Pontypool, Gwent NP4 OHZ</s2><s3>GBR</s3><sZ>5 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Pontypool, Gwent NP4 OHZ</wicri:noRegion></affiliation></author><author><name sortKey="Griffiths, John" sort="Griffiths, John" uniqKey="Griffiths J" first="John" last="Griffiths">John Griffiths</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Uniscan Instruments Ltd.</s1><s2>Buxton</s2><s3>GBR</s3><sZ>6 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Uniscan Instruments Ltd.</wicri:noRegion></affiliation></author><author><name sortKey="Jackson, Simon K" sort="Jackson, Simon K" uniqKey="Jackson S" first="Simon K." last="Jackson">Simon K. Jackson</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre for Research in Biomedicine, Faculty of Health and Life Sciences, University of the West of England</s1><s2>Bristol BS16 1QY</s2><s3>GBR</s3><sZ>3 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Bristol BS16 1QY</wicri:noRegion></affiliation></author><author><name sortKey="Hart, John P" sort="Hart, John P" uniqKey="Hart J" first="John P." last="Hart">John P. Hart</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Analytical, Materials and Sensors Science, Faculty of Health and Life Sciences, University of the West of England</s1><s2>Bristol BS16 1QY</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Bristol BS16 1QY</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Mikrochimica acta : (1966. Print)</title><title level="j" type="abbreviated">Mikrochim. acta : (1966. Print)</title><idno type="ISSN">0026-3672</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Mikrochimica acta : (1966. Print)</title><title level="j" type="abbreviated">Mikrochim. acta : (1966. Print)</title><idno type="ISSN">0026-3672</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amperometry</term><term>Application</term><term>Biosensor</term><term>Buffer solution</term><term>Carbon</term><term>Cell culture</term><term>Cobalt</term><term>Culture medium</term><term>Cyclic voltammetry</term><term>Diffusion</term><term>Dynamics</term><term>Environment</term><term>Enzyme</term><term>Glucose oxidase</term><term>Hexacyanoferrates II</term><term>High density</term><term>Ink</term><term>Lactates</term><term>Microelectrode</term><term>Monitoring</term><term>Phosphates</term><term>Phthalocyanine</term><term>Platinum</term><term>Production</term><term>Reduction</term><term>Release</term><term>Sensitivity</term><term>Steady state</term><term>Time</term><term>Toxicity</term><term>Toxin</term><term>Use</term><term>Water</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Application</term><term>Biodétecteur</term><term>Environnement</term><term>Toxine</term><term>Carbone</term><term>Cobalt</term><term>Enzyme</term><term>Solution tampon</term><term>Voltammétrie cyclique</term><term>Densité élevée</term><term>Régime permanent</term><term>Ampérométrie</term><term>Diffusion</term><term>Microélectrode</term><term>Eau</term><term>Encre</term><term>Phtalocyanine</term><term>Glucose oxidase</term><term>Lactate</term><term>Hexacyanoferrate II</term><term>Phosphate</term><term>Utilisation</term><term>Platine</term><term>Milieu culture</term><term>Sensibilité</term><term>Dynamique</term><term>Temps</term><term>Libération</term><term>Benzène(1,3-dinitro)</term><term>Réduction</term><term>Production</term><term>Culture cellulaire</term><term>Toxicité</term><term>Monitorage</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Carbone</term><term>Cobalt</term><term>Enzyme</term><term>Eau</term><term>Phosphate</term><term>Platine</term><term>Production</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferrocyanide and H<sub>2</sub>O<sub>2</sub> in phosphate buffer solution: sigmoidal cyclic voltammograms, high current density values and steady-state amperometric responses confirmed the existence of radial-diffusion-limiting microelectrode behaviour. The lactate microband biosensors were then used, in conjunction with a screen-printed Ag/AgCl reference and platinum counter electrode, to monitor lactate levels in culture medium, with a linear range of 0.5-5 mM, sensitivity of 20 nA.mM<sup>-1</sup>, and dynamic range up to >9 mM. The lactate microband biosensors could operate continuously in culture medium over extended times (up to 24 h) at 37°C. These biosensors were then applied to detect changes in lactate release from cultured cells in response to toxic challenge: m-dinitrobenzene (500 μM) caused a reduction in lactate production by high-passage number HepG2 single cells; D-galactosamine (20 mM) induced release of lactate by HepG2 spheroid cultures. This novel use of microband biosensors in cell culture has the potential for further application in toxicity monitoring, in both environmental and pharmaceutical areas.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Pemberton, Roy M" sort="Pemberton, Roy M" uniqKey="Pemberton R" first="Roy M." last="Pemberton">Roy M. Pemberton</name></noRegion><name sortKey="Drago, Guido A" sort="Drago, Guido A" uniqKey="Drago G" first="Guido A." last="Drago">Guido A. Drago</name><name sortKey="Griffiths, John" sort="Griffiths, John" uniqKey="Griffiths J" first="John" last="Griffiths">John Griffiths</name><name sortKey="Hart, John P" sort="Hart, John P" uniqKey="Hart J" first="John P." last="Hart">John P. Hart</name><name sortKey="Jackson, Simon K" sort="Jackson, Simon K" uniqKey="Jackson S" first="Simon K." last="Jackson">Simon K. Jackson</name><name sortKey="Jinsheng Xu" sort="Jinsheng Xu" uniqKey="Jinsheng Xu" last="Jinsheng Xu">JINSHENG XU</name><name sortKey="Pittson, Robin" sort="Pittson, Robin" uniqKey="Pittson R" first="Robin" last="Pittson">Robin Pittson</name><name sortKey="Rawson, Frankie J" sort="Rawson, Frankie J" uniqKey="Rawson F" first="Frankie J." last="Rawson">Frankie J. Rawson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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